Creates a subsampled OTU table(OTU表抽平)

分析模块通过随机抽样对OTU Table抽平,生成样本序列数一致的OTU Table。序列数小于设定阈值的样本在输出结果中将会被忽略。

输入:

OTU Table文件:

OTU ID     Bio1 Bio2 Bio3 Bio4 Bio5 Bio6 Bio7 Bio8 Bio9 Bio10

OTU1        0       0       0       0       0       6       34     104  367  254

OTU2        52     335  18     49     0       0       0       0       0       0

OTU3        0       0       0       0       5       0       0       0       0       0

其他参数默认。

输出:

抽平后的OTU Table文件:

OTU ID     Bio1 Bio2 Bio3 Bio4 Bio5 Bio6 Bio7 Bio8 Bio9 Bio10

OTU1        0       0       0       0       0       6       34     70     235  205

OTU2        31     264  18     32     0       0       0       0       0       0

OTU3        0       0       0       0       4       0       0       0       0       0

分析模块引用了mothurv.1.35.0)的sub.sample命令。

相关文献如下所示:

Schloss, P.D., et al., Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol, 2009. 75(23):7537-41.

The original tools:

Schloss, PD & Handelsman, J. (2006) Introducing SONS, a tool for OTU-based comparisons of membership and structure between microbial communities. Applied and Environmental Microbiology. 72:6773-9.

Schloss, PD & Handelsman, J. (2006). Introducing TreeClimber, a test to compare community structures. Applied and Environmental Microbiology. 72: 2379-84.

Schloss, PD & Handelsman, J. (2005). Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Applied and Environmental Microbiology. 71:1501-6.

Schloss, PD, Larget, BR, & Handelsman J. (2004). Integration of microbial ecology and statistics: a test to compare gene libraries. Applied and Environmental Microbiology. 70:5485-92.