NT Annotation with BLAST+ blastn(NT数据库注释)
NT Annotation with BLAST+ blastn(NT数据库注释)

功能简介

分析模块,利用blastn程序,输入Fasta格式的核酸序列文件,与NT数据库进行比对,输出NT数据库注释结果。

注意

数据库比对需要大量的时间,如果输入的序列条数过多,会导致blast程序进入假死状态。建议,每次输入的序列数目不要太多。如果,序列条数过多,将序列文件切割成多个小文件,进行分析。


输出格式

ColumnNCBI nameDescription
1ScoreScore
2E-ValueE-Value
3HSP-LenLength of Match
4%-IdentPercentage of Identical Bases in Match
5%-SimilPercentage of Similar Bases in Match
6Query-NameQuery Name
7Num-Rds---
8Q-LenTotal Length of Query Sequence
9Q-BeginStarting Position of Match in Query
10Q-EndEnding Position of Match in Query
11Q-FrameQuery Frame
12Hit-NameHit Name
13H-LenTotal Length of Hit Sequence
14H-BeginStarting Position of Match in Hit
15H-EndEnding Position of Match in Hit
16H-FrameHit Frame
17DescriptionHit Description

分析模块,引用了 ncbi-blast-2.2.31+ 软件(ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.2.31/)

相关文献如下所示:

Camacho, Christiam and Coulouris, George and Avagyan, Vahram and Ma, Ning and Papadopoulos, Jason and Bealer, Kevin and Madden, Thomas L (2009). BLAST+: architecture and applications. In BMC Bioinformatics, 10 (1), pp. 421.